cd47 ig like domain Search Results


goat anti mcd47  (R&D Systems)
94
R&D Systems goat anti mcd47
Goat Anti Mcd47, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/goat anti mcd47/product/R&D Systems
Average 94 stars, based on 1 article reviews
goat anti mcd47 - by Bioz Stars, 2026-03
94/100 stars
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cd47  (R&D Systems)
93
R&D Systems cd47
( A ) ATAC-Seq analysis for JUN and the hedgehog genes Gli1 and Ptch1 in scleroderma fibroblasts (SSCL), scleroderma fibroblasts after JUN knockout (JUN-KO) or under vismodegib, and normal skin fibroblasts. The promoter regions are highlighted with red boxes. n = 2. ( B ) ATAC-Seq analysis for the immune checkpoints <t>CD47</t> and PD-L1 and the interleukin IL-6 in scleroderma fibroblasts, scleroderma fibroblasts after JUN knockout or under vismodegib, and normal skin fibroblasts. The promoter regions are highlighted with red boxes. n = 2. ( C ) Heatmap of differential open chromatin regulatory elements characterized from ATAC-Seq. The color bar shows the relative ATAC-Seq signal ( Z score of normalized read counts) as indicated. Samples 1 and 2 in both groups are individual samples. n = 2. ( D ) Fibrosis-linked genes with a 5-fold decline in promoter accessibility after JUN knockout. n = 2. ( E ) p-JUN expression in pulmonary fibroblasts and scleroderma fibroblasts on a 70 kPa hydrogel or a regular polystyrene plastic dish. Two-sided t test. ** P < 0.01; *** P < 0.001. n = 4. Tukey’s multiple comparison test. Bars represent means with standard deviations.
Cd47, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd47/product/R&D Systems
Average 93 stars, based on 1 article reviews
cd47 - by Bioz Stars, 2026-03
93/100 stars
  Buy from Supplier
extracellular domain antibody  (R&D Systems)
93
R&D Systems extracellular domain antibody
( A ) ATAC-Seq analysis for JUN and the hedgehog genes Gli1 and Ptch1 in scleroderma fibroblasts (SSCL), scleroderma fibroblasts after JUN knockout (JUN-KO) or under vismodegib, and normal skin fibroblasts. The promoter regions are highlighted with red boxes. n = 2. ( B ) ATAC-Seq analysis for the immune checkpoints <t>CD47</t> and PD-L1 and the interleukin IL-6 in scleroderma fibroblasts, scleroderma fibroblasts after JUN knockout or under vismodegib, and normal skin fibroblasts. The promoter regions are highlighted with red boxes. n = 2. ( C ) Heatmap of differential open chromatin regulatory elements characterized from ATAC-Seq. The color bar shows the relative ATAC-Seq signal ( Z score of normalized read counts) as indicated. Samples 1 and 2 in both groups are individual samples. n = 2. ( D ) Fibrosis-linked genes with a 5-fold decline in promoter accessibility after JUN knockout. n = 2. ( E ) p-JUN expression in pulmonary fibroblasts and scleroderma fibroblasts on a 70 kPa hydrogel or a regular polystyrene plastic dish. Two-sided t test. ** P < 0.01; *** P < 0.001. n = 4. Tukey’s multiple comparison test. Bars represent means with standard deviations.
Extracellular Domain Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/extracellular domain antibody/product/R&D Systems
Average 93 stars, based on 1 article reviews
extracellular domain antibody - by Bioz Stars, 2026-03
93/100 stars
  Buy from Supplier

Image Search Results


( A ) ATAC-Seq analysis for JUN and the hedgehog genes Gli1 and Ptch1 in scleroderma fibroblasts (SSCL), scleroderma fibroblasts after JUN knockout (JUN-KO) or under vismodegib, and normal skin fibroblasts. The promoter regions are highlighted with red boxes. n = 2. ( B ) ATAC-Seq analysis for the immune checkpoints CD47 and PD-L1 and the interleukin IL-6 in scleroderma fibroblasts, scleroderma fibroblasts after JUN knockout or under vismodegib, and normal skin fibroblasts. The promoter regions are highlighted with red boxes. n = 2. ( C ) Heatmap of differential open chromatin regulatory elements characterized from ATAC-Seq. The color bar shows the relative ATAC-Seq signal ( Z score of normalized read counts) as indicated. Samples 1 and 2 in both groups are individual samples. n = 2. ( D ) Fibrosis-linked genes with a 5-fold decline in promoter accessibility after JUN knockout. n = 2. ( E ) p-JUN expression in pulmonary fibroblasts and scleroderma fibroblasts on a 70 kPa hydrogel or a regular polystyrene plastic dish. Two-sided t test. ** P < 0.01; *** P < 0.001. n = 4. Tukey’s multiple comparison test. Bars represent means with standard deviations.

Journal: JCI Insight

Article Title: CD47 prevents the elimination of diseased fibroblasts in scleroderma

doi: 10.1172/jci.insight.140458

Figure Lengend Snippet: ( A ) ATAC-Seq analysis for JUN and the hedgehog genes Gli1 and Ptch1 in scleroderma fibroblasts (SSCL), scleroderma fibroblasts after JUN knockout (JUN-KO) or under vismodegib, and normal skin fibroblasts. The promoter regions are highlighted with red boxes. n = 2. ( B ) ATAC-Seq analysis for the immune checkpoints CD47 and PD-L1 and the interleukin IL-6 in scleroderma fibroblasts, scleroderma fibroblasts after JUN knockout or under vismodegib, and normal skin fibroblasts. The promoter regions are highlighted with red boxes. n = 2. ( C ) Heatmap of differential open chromatin regulatory elements characterized from ATAC-Seq. The color bar shows the relative ATAC-Seq signal ( Z score of normalized read counts) as indicated. Samples 1 and 2 in both groups are individual samples. n = 2. ( D ) Fibrosis-linked genes with a 5-fold decline in promoter accessibility after JUN knockout. n = 2. ( E ) p-JUN expression in pulmonary fibroblasts and scleroderma fibroblasts on a 70 kPa hydrogel or a regular polystyrene plastic dish. Two-sided t test. ** P < 0.01; *** P < 0.001. n = 4. Tukey’s multiple comparison test. Bars represent means with standard deviations.

Article Snippet: For immunohistochemistry/immunofluorescence we used adiponectin (Abcam, ab22554), CD3 (Abcam, ab5690), CD11b (Novus, NB110-89474), CD26 (Abcam, ab28340), CD26 (R&D Systems, Bio-Techne, AF954), CD31 (Dako, m0823), CD47 (Thermo Fisher Scientific, 14-0479-82, clone B6H12), CD47 (R&D Systems, Bio-Techne, AF1866), CD68 (Agilent, GA60691-2, clone KP1), cleaved caspase-3 (CST, 96645, clone 5A1E), FSP1 (MilliporeSigma, 07-2274), FSP1 (Abcam, ab58597), collagen 1 (Abcam, ab34710), FSP1 (MilliporeSigma, 07-2274), Ki67 (Abcam, ab15580), PD-1 (Cell Marque, 315M-96, clone NAT105), PD-1 (R&D Systems, Bio-Techne, AF1021), PD-L1 (R&D Systems, Bio-Techne, AF1019), and phospho–c-Jun (Ser73) (CST, 32705, clone D47G9).

Techniques: Knock-Out, Expressing, Comparison

( A ) Immunofluorescence stains against CD47 and FSP1 with and without JUN induction. Scale bar: 25 μm. n = 5. ( B ) Histogram of CD47 expression in fibroblasts with and without JUN induction. n = 5. ( C ) Percentage of CD47 positivity in different fibroblast populations with and without JUN induction. Fisher’s multiple comparison test. ** P < 0.01; *** P < 0.01. n = 5. Bars represent means with standard deviations. ( D ) Representative optical images of ectopically transplanted JUN-inducible mouse dermal fibroblasts ± CD47 inhibition. n = 4. ( E ) Corresponding quantification of photon emissions. Values are normalized to day 0. Fisher’s multiple comparison test. ** P < 0.01. n = 4. Bars represent means with standard deviations. ( F ) Fluorescent graft visualization under the dissection microscope after 7 days of CD47 inhibition. Scale bar: 5 mm. n = 2. ( G ) FACS plot for PE/RFP + CD11b + macrophages ± JUN induction ± CD47 inhibition in an in vitro phagocytosis assay. n = 3. ( H ) Corresponding quantification of RFP + macrophages. Tukey’s multiple comparison test. * P < 0.05; *** P < 0.01. n = 3. Bars represent means with standard deviations. ( I ) Schema of a macrophage depletion trial with subsequent skin fibrosis induction. n = 5. ( J ) Corresponding trichrome stains. Scale bar: 500 μm. n = 5. ( K ) Corresponding hydroxyproline assay. Two-sided t test. *** P < 0.001. n = 5. Bars represent means with standard deviations.

Journal: JCI Insight

Article Title: CD47 prevents the elimination of diseased fibroblasts in scleroderma

doi: 10.1172/jci.insight.140458

Figure Lengend Snippet: ( A ) Immunofluorescence stains against CD47 and FSP1 with and without JUN induction. Scale bar: 25 μm. n = 5. ( B ) Histogram of CD47 expression in fibroblasts with and without JUN induction. n = 5. ( C ) Percentage of CD47 positivity in different fibroblast populations with and without JUN induction. Fisher’s multiple comparison test. ** P < 0.01; *** P < 0.01. n = 5. Bars represent means with standard deviations. ( D ) Representative optical images of ectopically transplanted JUN-inducible mouse dermal fibroblasts ± CD47 inhibition. n = 4. ( E ) Corresponding quantification of photon emissions. Values are normalized to day 0. Fisher’s multiple comparison test. ** P < 0.01. n = 4. Bars represent means with standard deviations. ( F ) Fluorescent graft visualization under the dissection microscope after 7 days of CD47 inhibition. Scale bar: 5 mm. n = 2. ( G ) FACS plot for PE/RFP + CD11b + macrophages ± JUN induction ± CD47 inhibition in an in vitro phagocytosis assay. n = 3. ( H ) Corresponding quantification of RFP + macrophages. Tukey’s multiple comparison test. * P < 0.05; *** P < 0.01. n = 3. Bars represent means with standard deviations. ( I ) Schema of a macrophage depletion trial with subsequent skin fibrosis induction. n = 5. ( J ) Corresponding trichrome stains. Scale bar: 500 μm. n = 5. ( K ) Corresponding hydroxyproline assay. Two-sided t test. *** P < 0.001. n = 5. Bars represent means with standard deviations.

Article Snippet: For immunohistochemistry/immunofluorescence we used adiponectin (Abcam, ab22554), CD3 (Abcam, ab5690), CD11b (Novus, NB110-89474), CD26 (Abcam, ab28340), CD26 (R&D Systems, Bio-Techne, AF954), CD31 (Dako, m0823), CD47 (Thermo Fisher Scientific, 14-0479-82, clone B6H12), CD47 (R&D Systems, Bio-Techne, AF1866), CD68 (Agilent, GA60691-2, clone KP1), cleaved caspase-3 (CST, 96645, clone 5A1E), FSP1 (MilliporeSigma, 07-2274), FSP1 (Abcam, ab58597), collagen 1 (Abcam, ab34710), FSP1 (MilliporeSigma, 07-2274), Ki67 (Abcam, ab15580), PD-1 (Cell Marque, 315M-96, clone NAT105), PD-1 (R&D Systems, Bio-Techne, AF1021), PD-L1 (R&D Systems, Bio-Techne, AF1019), and phospho–c-Jun (Ser73) (CST, 32705, clone D47G9).

Techniques: Immunofluorescence, Expressing, Comparison, Inhibition, Dissection, Microscopy, In Vitro, Phagocytosis Assay, Hydroxyproline Assay

( A ) Schematic outline of the therapeutic trial. ( B ) Representative H&E and trichrome stains of the different groups. Scale bar: 500 μm. n = 4. ( C ) Hydroxyproline content of the skin. Tukey’s multiple comparison test. * P < 0.05; ** P < 0.01. n = 6. Graph bars represent means with standard deviations. ( D ) Amount of fat tissue. Values indicate μm 2 /μm skin width. Tukey’s multiple comparison test. * P < 0.05. n = 8. Graph bars represent means with standard deviations. ( E ) Representative optical images of ectopically transplanted GFP/luciferase-labeled human scleroderma fibroblasts ± CD47/IL-6 inhibition. ( F ) Optical imaging of explanted kidneys on day 5. ( G ) Quantification of photon emissions of explanted kidney grafts normalized to the values of the untreated mice. Two-sided t test. * P < 0.05. n = 3–4. Bars represent means with standard deviations. ( H ) Corresponding caspase-3 staining of kidney grafts. Scale bar: 25 μm. ( I ) Corresponding percentage of caspase-3 + GFP + fibroblasts. Two-sided t test. ** P < 0.01. n = 4–5. Bars represent means with standard deviations.

Journal: JCI Insight

Article Title: CD47 prevents the elimination of diseased fibroblasts in scleroderma

doi: 10.1172/jci.insight.140458

Figure Lengend Snippet: ( A ) Schematic outline of the therapeutic trial. ( B ) Representative H&E and trichrome stains of the different groups. Scale bar: 500 μm. n = 4. ( C ) Hydroxyproline content of the skin. Tukey’s multiple comparison test. * P < 0.05; ** P < 0.01. n = 6. Graph bars represent means with standard deviations. ( D ) Amount of fat tissue. Values indicate μm 2 /μm skin width. Tukey’s multiple comparison test. * P < 0.05. n = 8. Graph bars represent means with standard deviations. ( E ) Representative optical images of ectopically transplanted GFP/luciferase-labeled human scleroderma fibroblasts ± CD47/IL-6 inhibition. ( F ) Optical imaging of explanted kidneys on day 5. ( G ) Quantification of photon emissions of explanted kidney grafts normalized to the values of the untreated mice. Two-sided t test. * P < 0.05. n = 3–4. Bars represent means with standard deviations. ( H ) Corresponding caspase-3 staining of kidney grafts. Scale bar: 25 μm. ( I ) Corresponding percentage of caspase-3 + GFP + fibroblasts. Two-sided t test. ** P < 0.01. n = 4–5. Bars represent means with standard deviations.

Article Snippet: For immunohistochemistry/immunofluorescence we used adiponectin (Abcam, ab22554), CD3 (Abcam, ab5690), CD11b (Novus, NB110-89474), CD26 (Abcam, ab28340), CD26 (R&D Systems, Bio-Techne, AF954), CD31 (Dako, m0823), CD47 (Thermo Fisher Scientific, 14-0479-82, clone B6H12), CD47 (R&D Systems, Bio-Techne, AF1866), CD68 (Agilent, GA60691-2, clone KP1), cleaved caspase-3 (CST, 96645, clone 5A1E), FSP1 (MilliporeSigma, 07-2274), FSP1 (Abcam, ab58597), collagen 1 (Abcam, ab34710), FSP1 (MilliporeSigma, 07-2274), Ki67 (Abcam, ab15580), PD-1 (Cell Marque, 315M-96, clone NAT105), PD-1 (R&D Systems, Bio-Techne, AF1021), PD-L1 (R&D Systems, Bio-Techne, AF1019), and phospho–c-Jun (Ser73) (CST, 32705, clone D47G9).

Techniques: Comparison, Luciferase, Labeling, Inhibition, Optical Imaging, Staining